Premium
Modified crush‐avulsion anastomosis model on the rat femoral vein
Author(s) -
Fu Kaiding,
Izquierdo Ricardo,
Hubbard Thomas,
Fareed Jawed
Publication year - 1995
Publication title -
microsurgery
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.031
H-Index - 63
eISSN - 1098-2752
pISSN - 0738-1085
DOI - 10.1002/micr.1920160806
Subject(s) - medicine , avulsion , thrombus , heparin , saline , surgery , thrombosis , avulsion injury , anastomosis , vein , femoral vein , anesthesia
A crush‐avulsion anastomosis model was established on the rat femoral vein. Saline or heparin was used as a luminal topical agent and was allowed to contact the damaged endothelium for 10 min. Patency and coagulation parameters were investigated for 1 week. The heparin treated group had a patency rate of 93% at 1 hr vs. 13% for the saline treated group ( P <0.001). At 7 days, the heparin treated group had an 87% patency vs. 7% for the saline‐treated group ( P <0.001). Scanning electron micrography (SEM) provided evidence of the deposition of the components of early thrombosis in the crushed venous wall. In contrast, the SEM of the heparin treated group shows a paucity of any evidence of thrombus. These results indicate that the rat vein crush‐avulsion model is a reliable and reproducible thrombosis model with low patency. The methods used with the topical agent may improve the patency rate in crush avulsion injuries. © 1995 Wiley‐Liss, Inc.