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Modified trichrome staining technique of the nerve to determine proximal nerve viability
Author(s) -
Meyer Richard,
Claussen Gwendolyn C.,
Oh Shin J.
Publication year - 1995
Publication title -
microsurgery
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.031
H-Index - 63
eISSN - 1098-2752
pISSN - 0738-1085
DOI - 10.1002/micr.1920160302
Subject(s) - masson's trichrome stain , medicine , trichrome , staining , pathology , frozen section procedure , anatomy , trichrome stain , fibrosis , h&e stain , immunohistochemistry
Normal myelinated fibers are stained “red” by modified trichrome staining of the frozen section of the nerve. We have used this technique for rapid assessment of the anatomical integrity of the proximal nerve stump by identifying well‐preserved myelinated fibers. This technique can also identify degenerating and degenerated myelinated fibers, fibrosis, and inflammatory cells. We report three cases in which the practical usefulness of this technique is demonstrated. © 1995 Wiley‐Liss, Inc.