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Improved techniques for kidney transplantation in mice
Author(s) -
Zhang Zheng,
Schlachta Christopher,
Duff John,
Stiller Calvin,
Grant David,
Zhong Robert
Publication year - 1995
Publication title -
microsurgery
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.031
H-Index - 63
eISSN - 1098-2752
pISSN - 0738-1085
DOI - 10.1002/micr.1920160212
Subject(s) - medicine , anastomosis , cuff , ureter , transplantation , kidney transplantation , urinary system , kidney , renal vein , surgery , aorta , urinary bladder , inferior vena cava , urology , vein , anatomy
Improved microsurgical techniques for kidney transplantation in the mouse are described. Left renal transplantation is performed with end‐to‐side anastomoses of the donor renal vein to the inferior vena cava and the donor aortic cuff to the aorta. Urinary tract reconstruction is accomplished by suturing the donor ureter with a bladder patch to the recipient bladder. With these modifications, it is possible to achieve success rates as high as 90%. This stable and reproducible model provides a useful tool to study the immunological mechanisms of kidney allograft rejection at the molecular level. © 1995 Wiley‐Liss, Inc.