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Direct visualization and measurements of wound neovascularization: Application in microsurgery research
Author(s) -
Kjolseth Dorthe,
Kim Michael K.,
Andresen Louise H.,
Morsing Annie,
Frank Johannes M.,
Schuschke Dale,
Anderson Gary L.,
Banis Joseph C.,
Tobin Gordon R.,
Weiner Leonard J.,
Hjortdal Vibeke E.,
Barker John H.
Publication year - 1994
Publication title -
microsurgery
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.031
H-Index - 63
eISSN - 1098-2752
pISSN - 0738-1085
DOI - 10.1002/micr.1920150606
Subject(s) - neovascularization , medicine , hairless , wound healing , angiogenesis , microsurgery , surgery , pathology , biology , genetics
Abstract Neovascularization or angiogenesis is an essential yet poorly understood component of the healing process. In wound healing research, there is a lack of models enabling quantitative and continuous measurements of wound neovascul‐arization. The hairless mouse ear wound model permits quantitative measurements of wound epithelialization and neovascularization continuously throughout the healing process. On the ears of male homozygous (hr/hr) hairless mice, standardized circular full thickness dermal wounds are produced; then, using vital microscopy, these two processes are directly viewed and measured at day 0 and every third day thereafter until these are complete. This model system and its application to clinically relevant situations are reviewed. © 1994 Wiley‐Liss, Inc.