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Metabolic effect of pancreaticoduodenal transplantation in diabetic rats
Author(s) -
Spadella César T.,
Breim Luiz C.,
Mercadante Maria C. S.,
De Macedo Célia S.,
De Macedo Arthur R.
Publication year - 1992
Publication title -
microsurgery
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.031
H-Index - 63
eISSN - 1098-2752
pISSN - 0738-1085
DOI - 10.1002/micr.1920130307
Subject(s) - medicine , polyuria , polydipsia , endocrinology , pancreas transplantation , diabetes mellitus , transplantation , alloxan , glucagon , insulin , pancreas , urinary system , glycemic , kidney transplantation
Outbred Wistar rats were randomly assigned to three experimental groups: GI, 10 nondiabetic control rats; GII, 10 alloxan‐diabetic control rats; GIII, 25 alloxan‐diabetic rats that received pancreaticoduodenal transplantation (PDT) from normal donor Wistar rats and were immunosuppressed with cyclosporin A. For 7 prior and 4, 7, 14, 21, and 30 days posttransplantation (during which the animals were housed in metabolic cages for periods of 24 hours) body weight, water and food intake, urine output, blood and urinary glucose, plasma insulin, and glucagon were recorded. These parameters were also concurrently recorded for diabetic and nondiabetic control rats. Animals were sacrificed after 30 days and histological and immunohistochemical studies of the pancreas were performed. Pancreatic transplants consistently and significantly improved the metabolic abnormalities of the diabetic rat ( P <0.01) by restoring body weight gain, and by immediate relief of hyperglycemia, glucosuria, polyuria, polydipsia, and also the low levels of plasma insulin. The plasma glucagon, elevated in diabetic control rats, did not change after transplant. © 1992 Wiley‐Liss, Inc.