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Effects of simultaneous kidney‐pancreaticoduodenal transplantation on diabetes‐induced renal insufficiency in rats
Author(s) -
Yoon Jin Han,
Jung Se Il,
Cho Charles,
Gil Myung Cheol,
Kim Choon Gon
Publication year - 2001
Publication title -
microsurgery
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.031
H-Index - 63
eISSN - 1098-2752
pISSN - 0738-1085
DOI - 10.1002/micr.1034
Subject(s) - medicine , diabetes mellitus , kidney , transplantation , urology , creatinine , insulin , urinary system , surgery , aorta , kidney transplantation , endocrinology
Abstract An investigation of the functional and histological changes was done after en‐bloc kidney‐pancreaticoduodenal transplantation (kpdt) in the diabetes‐induced, renal insufficient Lewis rats. For donor preparation, an end‐to‐side portocaval shunt was performed, and the aortic, vena caval segments, and ureter‐bladder patch were obtained. They were anastomosed microsurgically to recipient's aorta, vena cava, and bladder in end‐to‐side fashion. Of 15 diabetes‐induced kpdt rats, 14 survived. Two of the 14 surviving rats showed ischemic necrosis. The remaining 12 transplants showed well‐preserved glomeruli and Langerhans islets for 5 months postoperatively. Biochemical data comparing diabetic and sham‐operated rats (six rats each), six diabetic controls, and 12 kpdt rats showed no significant statistical difference at said observation period. The diabetes‐induced kpdt rats showed improvement of following biochemical data: within 1 week postoperatively, the glucose level fell from 300 to 115 mg/dL; BUN level from >20 to <20 mg/dL; the creatinine level from 1.5 to <1.2 mg/dL. The insulin level returned to normal, 1.1 ng/mL, in 2 weeks. The results demonstrate that the kpdt model is an effective and successful operative technique in diabetic rats and may provide effective therapeutic methods for diabetes‐induced renal insufficiency. © 2001 Wiley‐Liss, Inc. MICROSURGERY 21:173–178 2001