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Comparison of in vivo lymphocyte proliferation between allogeneic and xenogeneic heart transplantation in mice
Author(s) -
Kellersmann Richard,
Kellersmann Anne,
Thiede Arnulf,
Ulrichs Karin
Publication year - 2003
Publication title -
microsurgery
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.031
H-Index - 63
eISSN - 1098-2752
pISSN - 0738-1085
DOI - 10.1002/micr.10183
Subject(s) - xenotransplantation , in vivo , medicine , splenocyte , immunology , bromodeoxyuridine , cd8 , transplantation , lymphocyte , immune system , cd19 , flow cytometry , mixed lymphocyte reaction , population , t cell , biology , immunohistochemistry , microbiology and biotechnology , environmental health
There are controversial in vitro data comparing the strength of the cellular immune response between allogeneic and xenogeneic stimulator/responder combinations. The present study therefore compares in vivo lymphocyte proliferation using heart transplantation (HTx) models in mice. Heterotopic HTx into BALB/c mice was performed using donor organs from mice (BALB/c and C57BL/6) or Lewis rats. Intraperitoneally given bromodeoxyuridine (BrdU) was incorporated into the DNA and was subsequently analyzed by flow cytometry. On postoperative days 3 and 5, proliferation of splenocytes, CD4 + T‐lymphocytes, and CD19 + B‐lymphocytes was significantly higher after xenogeneic than after allogeneic and isogeneic HTx. No significant difference was observed when proliferation of CD8 + lymphocytes was determined. The increased in vivo proliferation after xenotransplantation may reflect an earlier and probably stronger cellular immune response compared to allogeneic transplantation. The higher CD4 + lymphocyte proliferation underscores the importance of this cell population in xenograft rejection. © 2003 Wiley‐Liss, Inc. MICROSURGERY 23:498–502 2003