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Effects of liposome‐mediated gene transfer of VEGF in ischemic rat gracilis muscle
Author(s) -
Neumeister Michael W.,
Song YaoHua,
Mowlavi Arian,
Suchy Hans,
Mathur Aruna
Publication year - 2001
Publication title -
microsurgery
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.031
H-Index - 63
eISSN - 1098-2752
pISSN - 0738-1085
DOI - 10.1002/micr.1010
Subject(s) - vascularity , lipofectamine , medicine , gracilis muscle , vascular endothelial growth factor , ischemia , genetic enhancement , anatomy , andrology , surgery , vegf receptors , gene , biology , recombinant dna , biochemistry , vector (molecular biology)
The purpose of the current study was to determine the effects of vascular endothelial growth factor (VEGF) on muscle flap survival and vascularity in a rat gracilis ischemia‐reperfusion model. A total of 12 adult male Wistar rats were divided into two groups (n = 6). The experimental group received the plasmid encoding VEGF 165 cDNA plus lipofectamine (cationic liposome) injected directly to the gracilis muscle following 4 h of ischemia. The control group received lipofectamine only. The viability and vascularity of the flaps were evaluated after 7 days of reperfusion. The data demonstrated that the VEGF plasmid‐ and lipofectamine‐treated muscle flaps had significantly greater total survival and capillary count 7 days after reperfusion compared with the flaps treated only with lipofectamine. These results indicate that VEGF exerts a protective effect on ischemic skeletal muscle flaps. © 2001 Wiley‐Liss, Inc. MICROSURGERY 21:58–62 2001