Effects of liposome‐mediated gene transfer of VEGF in ischemic rat gracilis muscle | Zendy

Neumeister Michael W. | Zendy; Song YaoHua | Zendy; Mowlavi Arian | Zendy; Suchy Hans | Zendy; Mathur Aruna | Zendy

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Effects of liposome‐mediated gene transfer of VEGF in ischemic rat gracilis muscle

Author(s) -

Neumeister Michael W.,

Song YaoHua,

Mowlavi Arian,

Suchy Hans,

Mathur Aruna

Publication year - 2001

Publication title -

microsurgery

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.031

H-Index - 63

eISSN - 1098-2752

pISSN - 0738-1085

DOI - 10.1002/micr.1010

Subject(s) - vascularity , lipofectamine , medicine , gracilis muscle , vascular endothelial growth factor , ischemia , genetic enhancement , anatomy , andrology , surgery , vegf receptors , gene , biology , recombinant dna , biochemistry , vector (molecular biology)

The purpose of the current study was to determine the effects of vascular endothelial growth factor (VEGF) on muscle flap survival and vascularity in a rat gracilis ischemia‐reperfusion model. A total of 12 adult male Wistar rats were divided into two groups (n = 6). The experimental group received the plasmid encoding VEGF 165 cDNA plus lipofectamine (cationic liposome) injected directly to the gracilis muscle following 4 h of ischemia. The control group received lipofectamine only. The viability and vascularity of the flaps were evaluated after 7 days of reperfusion. The data demonstrated that the VEGF plasmid‐ and lipofectamine‐treated muscle flaps had significantly greater total survival and capillary count 7 days after reperfusion compared with the flaps treated only with lipofectamine. These results indicate that VEGF exerts a protective effect on ischemic skeletal muscle flaps. © 2001 Wiley‐Liss, Inc. MICROSURGERY 21:58–62 2001

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