Open AccessA comprehensive immune repertoire study for patients with pulmonary tuberculosis
Author(s) -
Fu Yingyun,
Li Bo,
Li Yazhen,
Wang Minlian,
Yue Yongjian,
Xu Lan,
Li Shulin,
Huang Qijun,
Liu Song,
Dai Yong
Publication year - 2019
Publication title -
molecular genetics and genomic medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.765
H-Index - 29
ISSN - 2324-9269
DOI - 10.1002/mgg3.792
Subject(s) - tuberculosis , repertoire , immunology , immune system , antibody , biology , gene , cd8 , medicine , genetics , pathology , physics , acoustics
Background Tuberculosis (TB) is a major global health problem and has replaced HIV as the leading cause of death from a single infectious agent. Methods Here, we applied high throughput sequencing to study the immune repertoire of nine pulmonary tuberculosis patients and nine healthy control samples. Results Tuberculosis patients and healthy controls displayed significantly different high express clones and distinguishable sharing of CDR3 sequences. The TRBV and TRBJ gene usage showed higher expression clones in patients than in controls and we also found specific high express TRBV and TRBJ gene clones in different groups. In addition, six highly expressed TRBV/TRBJ combinations were detected in the CD4 group, 21 in the CD8 group and 32 in the tissue group. Conclusion In conclusion, we studied the patients with tuberculosis as well as healthy control individuals in order to understand the characteristics of immune repertoire. Sharing of CDR3 sequences and differential expression of genes was found among the patients with tuberculosis which could be used for the development of potential vaccine and targets treatment.