Open AccessmiR‐584‐5p regulates hepatocellular carcinoma cell migration and invasion through targeting KCNE2
Author(s) -
Wei Huamei,
Wang Jianchu,
Xu Zuoming,
Lu Yuan,
Wu Xianjian,
Zhuo Chenyi,
Tan Chuan,
Tang Qianli,
Pu Jian
Publication year - 2019
Publication title -
molecular genetics and genomic medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.765
H-Index - 29
ISSN - 2324-9269
DOI - 10.1002/mgg3.702
Subject(s) - microrna , cancer research , cell migration , cell growth , biology , hepatocellular carcinoma , cell , gentamicin protection assay , cell culture , medicine , cancer , metastasis , gene , genetics
Background Hepatocellular carcinoma (HCC) is one of the most commonly diagnosed cancer type. This study was aimed to investigate the role of microRNA‐584‐5p ( miR‐584‐5p ) in regulating HCC progression. Methods The expression of miR‐584‐5p in HCC cell lines was analyzed by quantitative real‐time polymerase chain reaction. Effects of miR‐584‐5p depletion on HCC cell proliferation, migration, and invasion in vitro were analyzed by cell counting kit‐8 assay, wound‐healing assay, and transwell invasion assay. miR‐584‐5p targeting potassium voltage‐gated channel subfamily E regulatory subunit 2 ( KCNE2 ) was identified using bioinformatics algorithm and dual‐luciferase activity reporter assay. Kaplan–Meier Plotter website was used to investigate the effect of miR‐584‐5p or KCNE2 expression on the overall survival of HCC patients. Results In vitro functional assays showed miR‐584‐5p depletion decreased HCC cell proliferation, cell migration, and cell invasion. Moreover, miR‐584‐5p functions by directly targeting KCNE2 , and it in turn, mediates the effects of miR‐584‐5p on HCC cell behaviors. Conclusions These results demonstrated that miR‐584‐5p functions as an oncogenic miRNA in HCC.