Open AccessIRS 1‐ rs10498210 G/A and CCR 5 ‐59029 A/G polymorphisms in patients with type 2 diabetes in Kurdistan
Author(s) -
Keshavarzi Fatemeh,
Golsheh Shadi
Publication year - 2019
Publication title -
molecular genetics and genomic medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.765
H-Index - 29
ISSN - 2324-9269
DOI - 10.1002/mgg3.631
Subject(s) - genotype , type 2 diabetes , diabetes mellitus , restriction fragment length polymorphism , polymorphism (computer science) , irs1 , population , genetics , biology , endocrinology , medicine , gene , microbiology and biotechnology , insulin receptor , insulin resistance , environmental health
Background The insulin receptor substrate 1 ( IRS 1 ) is a critical factor in the signaling pathway for insulin, and mutations in this gene have been reported, which contribute to the ability to develop type 2 diabetes. The polymorphisms in the promoter region of C‐C motif chemokine receptor5 ( CCR 5 ) are also being studied as candidates for susceptibility to develop type 2 diabetes. The aim of the current study was to determine the relationship between IRS 1 and CCR 5 polymorphisms with type 2 diabetes in the Kurdistan population. Methods Genomic DNA was isolated from the blood by salt extraction method and the polymorphisms were examined using Restriction Fragment Length Polymorphism ( RFLP ) method. Results The results of current study indicated that the frequency of AA genotype in type 2 diabetic patients in both CCR 5 ( OR = 2.9, p = 0.04) and IRS 1 ( OR = 3.3, p = 0.036) were significantly more than controls. Conclusion According to the results of this study, the presence of AA genotype in both CCR 5 and IRS 1 is associated with type 2 diabetes. There was no significant association between AG or GG genotypes with type 2 diabetes.