Open Accessmicro RNA ‐27a and micro RNA ‐146a SNP in cerebral malaria
Author(s) -
Wah Saw Thu,
Hananantachai Hathairad,
Patarapotikul Jintana,
Ohashi Jun,
Naka Izumi,
Nuchnoi Pornlada
Publication year - 2019
Publication title -
molecular genetics and genomic medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.765
H-Index - 29
ISSN - 2324-9269
DOI - 10.1002/mgg3.529
Subject(s) - cerebral malaria , rna , single nucleotide polymorphism , biology , snp , genotype , malaria , genotyping , gene , gene expression , plasmodium falciparum , genetics , immunology
Background During Plasmodium falciparum infection, micro RNA expression alters in brain tissue of mice with cerebral malaria compared to noninfected controls. Micro RNA regulates gene expression post‐transcriptionally to influence biological processes. Cerebral malaria pathology caused mainly by the immunological disorder. We hypothesize that single‐nucleotide polymorphism in a micro RNA influences micro RNA biogenesis or target gene recognition and altering susceptibility to cerebral malaria. Methods We performed a literature search based on immunological mechanism and applied micro RNA ‐related single‐nucleotide polymorphisms database to examine candidate micro RNA SNP s possibly responsible for cerebral malaria. Micro RNA ‐27a and micro RNA ‐146a are supposed to involve in cerebral malaria pathology. To assess the relationship of micro RNA SNP to cerebral malaria outcome, we performed TaqMan Genotyping Assays in 110 cerebral malaria and 207 uncomplicated malaria cases for three candidate micro RNA SNP s (rs895819 of micro RNA ‐27a, rs57095329 and rs2910164 of micro RNA ‐146a). Results Our study detected no significant difference in genotype and allele frequency of individual micro RNA SNP s as well as in haplotypes of micro RNA ‐146a between these two groups of malaria patients in Thailand. Hardy–Weinberg disequilibrium of rs57095329 in the cerebral malaria group showed a heterozygous excess which might be due to natural selection. Conclusion Our data supported that the candidate micro RNA SNP s have no major role to develop cerebral malaria.