Open AccessMicroRNA‐451a acts as tumor suppressor in cutaneous basal cell carcinoma
Author(s) -
Sun Hui,
Jiang Pingdong
Publication year - 2018
Publication title -
molecular genetics and genomic medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.765
H-Index - 29
ISSN - 2324-9269
DOI - 10.1002/mgg3.473
Subject(s) - microrna , downregulation and upregulation , cell growth , cancer research , cell cycle , carcinogenesis , basal cell carcinoma , luciferase , cell , biology , g1 phase , cell culture , microbiology and biotechnology , cancer , cell cycle checkpoint , pathology , medicine , transfection , basal cell , gene , biochemistry , genetics
Background Basal cell carcinoma (BCC) is the most common type of skin cancer. The underlying mechanism leading to BCC formation is not fully uncovered. The aim of this study was to characterize miRNA‐451a as a novel tumor suppressor in cutaneous BCC. Methods We first evaluated miRNA‐451a level in human BCC clinical tissues and inducible BCC mouse model. Then we studied the impact of overexpressing or inhibiting miR‐451a in cell proliferation, colony formation potential, and cell cycle pattern. Next, we employed luciferase reporter assay and western blotting to evaluate TBX1 as a downstream target of miRNA‐451a. Lastly, we confirmed TBX1 expressional change in BCC tissues by qPCR. Results miRNA‐451a was significantly reduced in human BCC tissues. The downregulation of miRNA‐451a was also confirmed in BCC mouse model. Overexpressing miRNA‐451a in tumor cells markedly suppressed cell growth through G1 cell cycle arrest. However, inhibiting miRNA‐451a in primary cells promoted cell growth and colony formation capacity. TBX1 (602054) was predicted as a downstream target of miRNA‐451a and this was confirmed by luciferase assay and protein expression. Finally, TBX1 level was shown upregulated in BCC tissues as inversely to miR451a. Conclusion Our studies revealed that miRNA‐451a/ TBX1 axis played a pivotal role in BCC tumorigenesis.