Open AccessIdentification and functional annotation of metabolism‐associated lnc RNA s and their related protein‐coding genes in gastric cancer
Author(s) -
Mo Xiaoyan,
Li Tianwen,
Xie Yi,
Zhu Linwen,
Xiao Bingxiu,
Liao Qi,
Guo Junming
Publication year - 2018
Publication title -
molecular genetics and genomic medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.765
H-Index - 29
ISSN - 2324-9269
DOI - 10.1002/mgg3.427
Subject(s) - rna , biology , gene expression , gene , non coding rna , carcinogenesis , long non coding rna , microbiology and biotechnology , cancer , rna editing , cancer research , genetics
Background Long noncoding RNA s (lnc RNA s) play important roles in carcinogenesis. However, the roles of metabolism‐associated lnc RNA s in cancers are still unclear. Methods A microarray of metabolism‐associated lnc RNA s was used to detect their expression patterns between gastric cancer and paired nontumorous tissues. Its results and gastric cancer differential gene expression data from public databases were used to screen the metabolic pathway‐associated lnc RNA s. A metabolic network with micro RNA s (mi RNA s), lnc RNA s, and protein‐coding genes was further constructed. Finally, the expression of TOPORS antisense RNA 1 ( TOPORS ‐ AS 1), a screened highly expressed lnc RNA and its associated protein‐coding gene, NADH : ubiquinone oxidoreductase subunit B6 ( NDUFB 6), were verified by reverse transcription polymerase chain reaction. Results A total of eight upregulated and one downregulated lnc RNA s and 25 upregulated and 20 downregulated protein‐coding genes were found to be involved in metabolism in gastric cancer. Within the lnc RNA s–mi RNA s– mRNA s metabolic network, 78 mi RNA ‐target links, 546 positive coexpression relationships, and 191 protein–protein interactions were found. The expression of TOPORS ‐ AS 1 and its associated gene, NDUFB 6 in gastric cancer tissues was significantly lower than that in adjacent nontumor tissues. Moreover, NDUFB 6 expression was associated with the invasion and distal metastasis of gastric cancer. Conclusions The metabolism‐associated lnc RNA s play important roles in the occurrence of gastric cancer.