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Dental malformations associated with biallelic MMP20 mutations
Author(s) -
Wang ShihKai,
Zhang Hong,
Chavez Michael B.,
Hu Yuanyuan,
Seymen Figen,
Koruyucu Mine,
Kasimoglu Yelda,
Colvin Connor D.,
Kolli Tamara N.,
Tan Michelle H.,
Wang YinLin,
Lu PeiYing,
Kim JungWook,
Foster Brian L.,
Bartlett John D.,
Simmer James P.,
Hu Jan C.C.
Publication year - 2020
Publication title -
molecular genetics and genomic medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.765
H-Index - 29
ISSN - 2324-9269
DOI - 10.1002/mgg3.1307
Subject(s) - amelogenesis imperfecta , enamel paint , ameloblast , dentinogenesis imperfecta , dentin , chemistry , amelogenesis , odontoblast , amelogenin , molar , microbiology and biotechnology , gene , pathology , dentistry , biochemistry , biology , medicine
Background Matrix metallopeptidase 20 (MMP20) is an evolutionarily conserved protease that is essential for processing enamel matrix proteins during dental enamel formation. MMP20 mutations cause human autosomal recessive pigmented hypomaturation‐type amelogenesis imperfecta (AI2A2; OMIM #612529). MMP20 is expressed in both odontoblasts and ameloblasts, but its function during dentinogenesis is unclear. Methods We characterized 10 AI kindreds with MMP20 defects, characterized human third molars and/or Mmp20 −/− mice by histology, Backscattered Scanning Electron Microscopy (bSEM), µCT, and nanohardness testing. Results We identified six novel MMP20 disease‐causing mutations. Four pathogenic variants were associated with exons encoding the MMP20 hemopexin‐like (PEX) domain, suggesting a necessary regulatory function. Mutant human enamel hardness was softest (13% of normal) midway between the dentinoenamel junction (DEJ) and the enamel surface. bSEM and µCT analyses of the third molars revealed reduced mineral density in both enamel and dentin. Dentin close to the DEJ showed an average hardness number 62%–69% of control. Characterization of Mmp20 −/− mouse dentin revealed a significant reduction in dentin thickness and mineral density and a transient increase in predentin thickness, indicating disturbances in dentin matrix secretion and mineralization. Conclusion These results expand the spectrum of MMP20 disease‐causing mutations and provide the first evidence for MMP20 function during dentin formation.

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