Open AccessClinical performance of DNA‐based prenatal screening using single‐nucleotide polymorphisms approach in Thai women with singleton pregnancy
Author(s) -
Panchalee Tachjaree,
Poungvarin Naravat,
Amornrit Warisa,
Pooliam Julaporn,
Taluengjit Pattarawalai,
Wataganara Tuangsit
Publication year - 2020
Publication title -
molecular genetics and genomic medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.765
H-Index - 29
ISSN - 2324-9269
DOI - 10.1002/mgg3.1256
Subject(s) - amniocentesis , single nucleotide polymorphism , medicine , obstetrics , trisomy , monosomy , prenatal diagnosis , gestational age , pregnancy , cell free fetal dna , fetus , population , gynecology , genetics , karyotype , chromosome , biology , genotype , gene , environmental health
Background To review the performance of noninvasive prenatal screening (NIPS) using targeted single‐nucleotide polymorphisms (SNPs) approach in mixed‐risk Thai women. Methods Retrospective analysis of data for detection of trisomy 21 (T21), 18 (T18), 13 (T13), monosomy X (XO), other sex chromosome aneuploidies (SCA), and triploidy/vanishing twins (VT) from a single commercial laboratory. Results Mean (± SD ) gestational age and maternal weight were 13.2 (±2.1) weeks and 125.7 (±22.4) pounds, respectively ( n = 8,572). From 462/8,572 (5.4%) no‐calls; 1/462 (0.2%) was uninformative SNPs, and 1/462 chose amniocentesis. Redraw settled 323/460 (70%) samples with low fetal fraction (FF); and 8,434/8,572 (98.4%) were finally reportable, with 131 high risks (1.6%). The median (min‐max) FF of reportable ( n = 8,434) and unreportable samples ( n = 137) samples were 10.5% (2.6–37.9) and 3.8% (1–14.1), respectively ( p < .05). Fetal karyotypes were available in 106/131 (80.9%) and 52/138 (37.7%) high risk and repeated no‐calls, respectively. The positive predictive values (PPVs) for T21 ( n = 47), T18 ( n = 15), T13 ( n = 7), XO ( n = 8), other SCA ( n = 7), and triploidy/VT were 94%, 100%, 58.3%, 66.7%, 70%, and 57.1%, respectively. None of repeated no‐calls had aneuploidies. Conclusion SNP‐based NIPS has high PPVs for T21 and T18. Although the proprietary SNPs library is not population‐specific, uninformative SNPs are uncommon.