Open AccessMIR‐138‐5P inhibits the progression of prostate cancer by targeting FOXC1
Author(s) -
Huang Hui,
Xiong Ying,
Wu Zhensheng,
He Yuhui,
Gao Xianglin,
Zhou Zhangyan,
Wang Tao
Publication year - 2020
Publication title -
molecular genetics and genomic medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.765
H-Index - 29
ISSN - 2324-9269
DOI - 10.1002/mgg3.1193
Subject(s) - cancer research , oncogene , microrna , prostate cancer , cell growth , cell culture , cell , biology , suppressor , cancer , microbiology and biotechnology , cell cycle , gene , genetics
Abstract Background Studies have suggested that micro‐RNAs (miRNAs) can function as an oncogene or a tumor suppressor in cancers. However, the role of MIR‐138‐5P (613394) in prostate cancer (PCa) remains unclear. Methods Expression level of MIR‐138‐5P in PCa cell lines and normal cell line was analyzed with the quantitative real‐time PCR method. Cell counting kit‐8 assay, colony formation assay, wound‐healing assay, and transwell invasion assay were performed to analyze the biological functions of MIR‐138‐5P . Results We showed MIR‐138‐5P expression level was significantly decreased in PCa cell lines compared with the normal cell line. Overexpression of MIR‐138‐5P inhibits PCa cell proliferation, colony formation, cell migration, and cell invasion in vitro. Mechanistically, we showed Forkhead box C1 ( FOXC1 , 601090) was a direct target for MIR‐138‐5P in PCa. We confirmed that overexpression of FOXC1 partially reversed the effects of MIR‐138‐5P on PCa cell behaviors. Conclusions Collectively, we showed that MIR‐138‐5P functions as a tumor suppressor gene in PCa via targeting FOXC1 .