Open AccessEvaluation of allelic alterations in short tandem repeats in papillary thyroid cancer
Author(s) -
Dang Zhen,
Li Lu,
Kong Xia,
Zhang Guoan,
Liu Qi,
Li Haibin,
Li Liang,
Zhang Renya,
Cui Wen,
Wang Yequan
Publication year - 2020
Publication title -
molecular genetics and genomic medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.765
H-Index - 29
ISSN - 2324-9269
DOI - 10.1002/mgg3.1164
Subject(s) - loss of heterozygosity , microdissection , microsatellite , biology , pathology , locus (genetics) , laser capture microdissection , allele , thyroid cancer , tandem repeat , genetics , cancer , medicine , genome , gene , gene expression
Abstract Background Malignant tissue samples may be the only source of biological material for forensic investigations, including individual identification or paternity testing; however, such samples may lead to uncertainties due to frequent genomic aberrations associated with tumors, including alterations of the short tandem repeat (STR) loci used for forensic casework. Methods Short tandem repeat loci routinely used in forensic analysis ( n = 23) were analyzed in 68 surgically removed papillary thyroid cancer specimens. Tumor cells and normal stromal cells were separated by laser capture microdissection. Results Four kinds of changes were detected between normal and tumor tissues: partial loss of heterozygosity (pLOH), complete loss of heterozygosity, an additional allele, and a new allele not found in normal tissue. These changes were distributed across 20 of the tested STRs, with no mutations in VWA, D16S539, or Penta D. The most frequently affected locus was D2S1338, and the most frequent type of alteration was pLOH. Samples from patients aged 40–59 years exhibited the highest frequencies of STR variation. Conclusion Our results suggest that great care should be taken in the evaluation of DNA typing results obtained from malignant tissues, particularly when no normal tissue reference samples are available.