Open AccessCirculating miR‐130b‐ and miR‐21‐based diagnostic markers and therapeutic targets for hepatocellular carcinoma
Author(s) -
Zhang Nannan,
Hu Zhenni,
Qiang Yong,
Zhu Xiaochao
Publication year - 2019
Publication title -
molecular genetics and genomic medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.765
H-Index - 29
ISSN - 2324-9269
DOI - 10.1002/mgg3.1012
Subject(s) - hepatocellular carcinoma , medicine , in vivo , downregulation and upregulation , microrna , liver cancer , cancer , therapeutic effect , cancer research , oncology , pathology , biology , gene , biochemistry , microbiology and biotechnology
Background Hepatocellular carcinoma ( HCC ) is one of the histological types of primary liver cancer with high recurrence and mortality in the world. The purpose of this study was to explore the diagnostic and therapeutic value for HCC patients. Methods In this study, we investigated the circulating miR‐130b‐5p (miR‐130b) and miR‐21‐5p (miR‐21) expression levels in patients with HCC and their association with clinical parameters. Results The circulating miR‐130b and miR‐21 were all upregulated in patients with HCC . The upregulated micro RNA s (mi RNA s) were associated with clinicopathological parameters of tumor capsular infiltration and clinical TNM stage. Also, the poor prognosis of patients with upregulated mi RNA s levels suggested that it may be an effective therapeutic target for HCC by suppression of the mi RNA s levels. In addition, the combined detection of serum miR‐130b and miR‐21 performed better in the diagnosis of HCC with a sensitivity of 92.16% and an accuracy rate of 77.51%. In vivo, tumors treated with the nanoparticle ( NP )/miR‐130b and miR‐21 inhibitor complexes had significantly lower growth than the other groups. Conclusion The circulating miR‐130b and miR‐21 can be used as potential tumor biomarkers to diagnose liver cancer, and the combined detection of serum miR‐130b and miR‐21 is superior to the diagnosis of HCC . NP /miR‐130b and miR‐21 inhibitor complexes show good therapeutic effects in vivo and are expected to become therapeutic targets worthy of further study.