Open AccessGraphene‐dispersed silane compound used as a coating to sense immunity from biofilm formation
Author(s) -
Kanematsu Hideyuki,
Nakagawa Ryoichi,
Sano Katsuhiko,
Barry Dana M.,
Ogawa Akiko,
Hirai Nobumitsu,
Kogo Takeshi,
Kuroda Daisuke,
Wada Noriyuki,
Lee SeungHyo,
Mizunoe Yoshimitsu
Publication year - 2019
Publication title -
medical devices & sensors
Language(s) - English
Resource type - Journals
ISSN - 2573-802X
DOI - 10.1002/mds3.10043
Subject(s) - graphene , biofilm , crystal violet , materials science , substrate (aquarium) , silane , biofouling , raman spectroscopy , exfoliation joint , chemical engineering , nanotechnology , chemical vapor deposition , coating , chemistry , composite material , bacteria , microbiology and biotechnology , optics , biochemistry , oceanography , physics , engineering , membrane , biology , geology , genetics
Biofilm is a substance that contains organic polymers. It is derived from bacteria, produces slime and forms on material's surfaces. Therefore, it is important to properly evaluate biofilm formation using several types of sensors. Since biofilm forms easily on graphene, it is possible for graphene to serve as a sensor for biofilm formation. In the past, graphene's sensitivity to biofilm formation was confirmed by using CVD (chemical vapour deposition) graphene and by using the tape‐exfoliation method. However, the CVD method was expensive and required special equipment. On the other hand, the exfoliation method was not so expensive, but the precision and reproducibility for graphene formation were problematic. In this experiment, silane‐based resin, with dispersed graphene, was coated on a substrate. This coated substrate can easily be used as a sensor and is an inexpensive way to promote biofilm formation. Glass was used as the substrate, for which various samples were prepared using different concentrations of dispersed graphene. Biofilms formed on the specimens’ surfaces were analysed by using Raman spectroscopy and the crystal violet staining method. The results showed that biofilm formation capability increased with the concentration of dispersed graphene. We confirmed that the sensitivity was evaluated by the colour value for stained surfaces by crystal violet.