Open AccessRecent advances and application of whole genome amplification in molecular diagnosis and medicine
Author(s) -
Wang Xiaoyu,
Liu Yapeng,
Liu Hongna,
Pan Wenjing,
Ren Jie,
Zheng Xiangming,
Tan Yimin,
Chen Zhu,
Deng Yan,
He gyue,
Chen Hui,
Li Song
Publication year - 2022
Publication title -
medcomm
Language(s) - English
Resource type - Journals
ISSN - 2688-2663
DOI - 10.1002/mco2.116
Subject(s) - genome , computational biology , dna sequencing , multiple displacement amplification , biology , single cell sequencing , whole genome sequencing , human genome , dna , genetics , polymerase chain reaction , gene , dna extraction , exome sequencing , mutation
Whole genome amplification (WGA) is a technology for non‐selective amplification of the whole genome sequence, first appearing in 1992. Its primary purpose is to amplify and reflect the whole genome of trace tissues and single cells without sequence bias and to provide sufficient DNA template for subsequent multigene and multilocus analysis, along with comprehensive genome research. WGA provides a method to obtain a large amount of genetic information from a small amount of DNA and provides a valuable tool for preserving limited samples in molecular biology. WGA technology is especially suitable for forensic identification and genetic disease research, along with new technologies such as next‐generation sequencing (NGS). In addition, WGA is also widely used in single‐cell sequencing. Due to the small amount of DNA in a single cell, it is often unable to meet the amount of samples needed for sequencing, so WGA is generally used to achieve the amplification of trace samples. This paper reviews WGA methods based on different principles, summarizes both amplification principle and amplification quality, and discusses the application prospects and challenges of WGA technology in molecular diagnosis and medicine.