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Abnormalities of the RB1 and DCC tumor suppressor genes: Uncommon in human pancreatic adenocarcinoma
Author(s) -
Barton Claire M.,
McKie Arthur B.,
Hogg Annette,
Bia Brita,
Elia George,
Phillips Stewart M. A.,
Ding ShiFa,
Lemoine Nicholas R.
Publication year - 1995
Publication title -
molecular carcinogenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.254
H-Index - 97
eISSN - 1098-2744
pISSN - 0899-1987
DOI - 10.1002/mc.2940130202
Subject(s) - biology , carcinogenesis , tumor suppressor gene , cancer research , pancreatic cancer , adenocarcinoma , allele , gene , suppressor , polymerase chain reaction , reverse transcription polymerase chain reaction , microbiology and biotechnology , cancer , gene expression , genetics
Evidence of RB1 allele loss was found in only 6% of pancreatic cancers, and we found no significant sequence abnormalities nor loss of RB protein expression in a panel of tumors and cell lines. Using reverse transcription‐polymerase chain reaction and Southern blot analysis, we found no evidence for loss of DCC expression in pancreatic cancer cell lines, and allele loss only rarely in tumor biopsies. These findings suggest that abnormalities of RB1 and DCC are unlikely to play a major role in pancreatic carcinogenesis. © 1995 Wiley‐Liss, Inc.