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Combined effects of ionizing radiation and cycloheximide on gene expression
Author(s) -
Woloschak Gayle E.,
Felcher Paolo,
ChangLiu ChinMei
Publication year - 1995
Publication title -
molecular carcinogenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.254
H-Index - 97
eISSN - 1098-2744
pISSN - 0899-1987
DOI - 10.1002/mc.2940130108
Subject(s) - biology , ionizing radiation , cycloheximide , gene , genetics , gene expression , microbiology and biotechnology , computational biology , protein biosynthesis , irradiation , physics , nuclear physics
We performed experiments to determine the effects of ionizing radiation exposure on expression of genes such as β‐actin, c‐ fos , histone H4, c‐ myc , c‐j un , Rb, and p53 after exposure of Syrian hamster embryo (SHE) cells to the protein synthesis inhibitor cycloheximide. The purpose of these experiments was to determine the role of a labile protein in the radiation‐induced response. The results revealed that when ionizing radiation (either fission‐spectrum neutrons or γ rays) was administered 15 min after cycloheximide treatment of SHE cells, the radiation exposure reduced cycloheximide‐mediated gene induction of c‐ fos , histone H4, and c‐ jun . In addition, dose‐rate differences were found when radiation exposure most significantly inhibited the cycloheximide response. Our results suggest that ionizing radiation does not act as a general protein‐synthesis inhibitor and that the presence of a labile protein is required for the maintenance of specific gene transcription and mRNA accumulation after radiation exposure, especially at high dose‐rates. © 1995 Wiley‐Liss, Inc.