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Low frequency of retinoblastoma gene alterations in rat hepatocellular carcinomas
Author(s) -
Smith Martin L.,
Lombardi Benito,
Chandar Nalini
Publication year - 1993
Publication title -
molecular carcinogenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.254
H-Index - 97
eISSN - 1098-2744
pISSN - 0899-1987
DOI - 10.1002/mc.2940080405
Subject(s) - hccs , biology , retinoblastoma , hepatocellular carcinoma , gene , gene product , cancer research , retinoblastoma protein , blot , gene expression , tumor suppressor gene , microbiology and biotechnology , carcinogenesis , cell cycle , genetics
Abnormalities of the retinoblastoma ( Rb ) gene have been reported in some human cancers, including hepatocellular carcinomas (HCCs). We examined by Southern blotting the status of the Rb gene in HCCs induced in rats in four experimental models. A low frequency of Rb gene alterations, detected as novel hybridizing bands unique to each tumor, was observed. Expression of the Rb protein product was examined in the HCCs and in seven established rat hepatoma cell lines studied. It appears, therefore, that alterations in the structure or expression of the Rb gene do occur but probably do not contribute in a major way to hepatocarcinogenesis in the rat. © 1993 Wiley‐Liss, Inc.