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High expression of cytochrome P450 2a‐5 (coumarin 7‐hydroxylase) in mouse hepatomas
Author(s) -
Kobliakov Valery,
Kulikova Ludmila,
Samoilov Dimitri,
Lang Matti A.
Publication year - 1993
Publication title -
molecular carcinogenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.254
H-Index - 97
eISSN - 1098-2744
pISSN - 0899-1987
DOI - 10.1002/mc.2940070411
Subject(s) - biology , cytochrome p450 , isozyme , coumarin , inducer , hydroxylation , biochemistry , enzyme , microbiology and biotechnology , medicine , endocrinology , gene , botany
A high level of Cyp2a‐5 was found in spontaneous and transplanted mouse hepatomas compared with normal liver. Increased expression of Cyp2a‐5 was associated with an increase in coumarin 7‐hydroxylation, a marker activity of Cyp2a‐5, and the corresponding mRNA, suggesting that regulation of Cyp2a‐5 in hepatomas is pretranslational. In contrast, the total P450 content and arylhydrocarbon hydroxylase and amidopyrene demethylase activities decreased. Pyrazole, a strong inducer of Cyp2a‐5 in normal mouse livers, also increases this isozyme in hepatomas. A parallel increase in the corresponding mRNA suggests that pyrazole, like the formation of hepatomas, affects the regulation of Cyp2a‐5 pretranslationally.