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A revised map position for the Ha‐ ras gene on mouse chromosome 7: Implications for analysis of genetic alterations in rodent tumors
Author(s) -
Kemp Christopher J.,
Bremner Rod,
Balmain Allan
Publication year - 1993
Publication title -
molecular carcinogenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.254
H-Index - 97
eISSN - 1098-2744
pISSN - 0899-1987
DOI - 10.1002/mc.2940070304
Subject(s) - biology , gene , genetics , chromosome , rodent , position (finance) , gene mapping , evolutionary biology , computational biology , ecology , finance , economics
Abstract The mouse Ha‐ ras gene has previously been mapped to the central region of chromosome 7, 31 cM from the centromere, using an interspecific Mus musculus/Mus spretus backcross (Saunders AM, Seldin MF, Genomics 8:525–535, 1990). However, analysis of mitotic recombinations in mouse skin tumors from intraspecific F 1 hybrid mice suggested a more distal location for the Ha‐ ras gene on chromosome 7 (Bremner R, Balmain A, Cell 61:407–417, 1990). In the study reported here, we demonstrated, by analysis of Ha‐ ras gene mutations in skin tumors from interspecific M. spretus/M. musculus F 1 hybrids, the existence only in M. spretus of a pseudogene or other Ha‐ ras —related sequence that is probably the sequence originally mapped by Saunders and Seldin. The functional Ha‐ ras gene maps to the distal region of chromosome 7, and it is this sequence that acquires mutations in chemically induced tumors.