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Quantitation of 2‐amino‐3‐methylimidazo[4,5‐ f ]quinoline and 2‐amino‐3,8‐dimethylimidazo[4,5‐ f ]quinoxaline DNA adducts in specific sequences using alkali or uvrABC excinuclease
Author(s) -
Nouso Kazuhiro,
Bohr Vilhelm A.,
Schut Herman A. J.,
Snyderwine Elizabeth G.
Publication year - 1993
Publication title -
molecular carcinogenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.254
H-Index - 97
eISSN - 1098-2744
pISSN - 0899-1987
DOI - 10.1002/mc.2940070211
Subject(s) - biology , dna , adduct , biochemistry , mutagen , carcinogen , microbiology and biotechnology , chemistry , organic chemistry
2‐Amino‐3‐methylimidazo[4,5‐ f ]quinoline (IQ) and 2‐amino‐3,8‐dimethylimidazo[4,5‐ f ]quinoxaline (MelQx) are carcinogens found in cooked meats that form DNA adducts upon metabolic activation. Purified DNA from Chinese hamster ovary (CHO) cells was reacted in vitro with the active metabolites N ‐acetoxy‐lQ or N ‐acetoxy‐MelQx, and the adduct levels in the 5′ dihydrofolate reductase ( DHFR ) gene and downstream region were quantitated by Southern hybridization. Adducted and restricted DNA was treated with Escherichia coli uvrABC excinuclease or alkali (0.1 N NaOH, 37°C, 60 min) to incise DNA at IQ and MeIQx adduct sites. The DNA was then denatured with formamide, electrophoresed on a neutral agarose gel, transferred to a support membrane, and hybridized with sequence‐specific DNA probes. Both uvrABC and alkali reduced the intensity of Southern hybridization in proportion to the number of IQ or MeIQx adducts in DNA, indicating that these adducts are substrates for uvrABC and that they form alkali‐labile lesions in DNA. IQ and MeIQx adduct levels were the same in the 5′ DHFR gene and in the downstream region. Southern hybridization analysis of pBR322 containing known levels of IQ or MeIQx adducts showed that the efficiency of cutting IQ or MeIQx adducts by uvrABC excinuclease and alkali was approximately 30% and 15%, respectively. 32 P‐postlabeling studies examining adduct level in bulk DNA further showed that the adduct profiles were identical in pBR322, CHO DNA, and cultured CHO cells exposed to the reactive metabolites of IQ or MeIQx. The results indicate that IQ and MeIOx adducts can be quantitated in specific genomic sequences and that this method should be directly applicable to studies of gene‐specific repair of these adducts in cultured cells.