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Differential retention of tumor‐ and differentiation‐suppressor functions in cells derived from a human squamous cell carcinoma
Author(s) -
Jaffe Deborah R.,
MonteroPuerner Yvonne,
Beckett Michael A.,
Cowan Janet M.,
Weichselbaum Ralph R.,
Diamond Alan M.
Publication year - 1992
Publication title -
molecular carcinogenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.254
H-Index - 97
eISSN - 1098-2744
pISSN - 0899-1987
DOI - 10.1002/mc.2940050408
Subject(s) - biology , suppressor , basal cell , cancer research , differential (mechanical device) , microbiology and biotechnology , medicine , cancer , genetics , engineering , aerospace engineering
Three morphologically distinct cell lines—F.2a, V, and B.2—were isolated from a single human squamous cell carcinoma. Although all three cell lines can grow indefinitely in culture, they differ in a number of important transformation‐related phenotypes. Only B.2 is strongly tumorigenic when injected into the flanks of nude mice, and only V can efficiently grow in semisolid media. The dominance of these traits was investigated by generating somatic cell hybrids among the three cell lines. F.2a was able to suppress the tumorigenicity of B.2 cells, whereas B.2 inhibited the capacity for anchorage‐independent growth of V, the latter trait being a function of the ability of these epithelial cells to differentiate when deprived of support. The influence of exogenously added growth factors was also evaluated. This study indicates that the particular tumor we examined consisted of a heterogeneous population of cells with distinct growth and differentiation capacities. © 1992 Wiley‐Liss, Inc.