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Characterization of the syrian hamster c‐Ha‐ ras gene and intron‐d‐exon transcript
Author(s) -
Ebert Rolf,
Wiseman Roger W.,
Barrett. J. Carl,
Reiss Eva,
Rollich Günther,
Schiffmann Deitmar
Publication year - 1992
Publication title -
molecular carcinogenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.254
H-Index - 97
eISSN - 1098-2744
pISSN - 0899-1987
DOI - 10.1002/mc.2940050404
Subject(s) - biology , intron , exon , microbiology and biotechnology , gene , complementary dna , coding region , alternative splicing , rna splicing , homology (biology) , genetics , genomic dna , nucleic acid sequence , hamster , rna
The coding sequences as well as 5′‐ and 3′‐flanking sequences of the Syrian hamster c‐Ha‐ ras gene were deduced from cDNA clones derived from embryo fibroblast cell lines. Sequences of introns B, C, and D were obtained from genomic DNA after amplification by the polymerase chain reaction. Sequence comparisons with rat, mouse, and human c‐Ha‐ ras genes revealed a high degree of homology. One of 12 cDNA clones contained intron‐D‐exon (IDX) sequences due to alternative splicing that would encode a p19 Ha‐ras gene product. Conservation between species suggests a functional role for the IDX, possibly as a negative control of p21 Ha‐ras expression. © 1992 Wiley‐Liss, Inc.