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Chromatin and cell surface receptors mediate melanoma cell growth response to nerve growth factor
Author(s) -
RakowiczSzulczynska Ewa M.,
Reddy Usha,
Vorbrodt Andrzej,
Herlyn Dorothee,
Koprowski Hilary
Publication year - 1991
Publication title -
molecular carcinogenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.254
H-Index - 97
eISSN - 1098-2744
pISSN - 0899-1987
DOI - 10.1002/mc.2940040511
Subject(s) - biology , nerve growth factor , chromatin , receptor , microbiology and biotechnology , cell culture , cell growth , cell , biochemistry , dna , genetics
Growth response to nerve growth factor (NGF) was tested in the primary melanoma cell line WM 164, which expressed a low level of NGF cell‐surface receptor, and in WM 164 cells transfected with cDNA for the cell‐surface receptor (TrWM 164 cells), which expressed a higher level of the cell‐surface receptor. Neither cell line expressed the chromatin receptor for NGF or internalized NGF. Both cell lines were stimulated to growth by NGF. After 10 d of exposure to NGF, a 230 000 M r chromatin protein (receptor) was induced in both cell lines; as a result, NGF bound to the chromatin, and ribosomal RNA synthesis and cell proliferation were inhibited. We suggest that the cell‐surface and chromatin receptors each mediate a different function of NGF.