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Stable expression of human cytochrome P450IA2 cDNA in a human lymphoblastoid cell line: Role of the enzyme in the metabolic activation of aflatoxin B 1
Author(s) -
Crespi Charles L.,
Steimel Dorothy T.,
Aoyama Toshifumi,
Gelboin Harry V.,
Gonzalez Frank J.
Publication year - 1990
Publication title -
molecular carcinogenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.254
H-Index - 97
eISSN - 1098-2744
pISSN - 0899-1987
DOI - 10.1002/mc.2940030104
Subject(s) - biology , aflatoxin , complementary dna , cell culture , microbiology and biotechnology , recombinant dna , lymphoblast , enzyme , cell , carcinogen , biochemistry , mycotoxin , gene , genetics , botany
A human lymphoblastoid cell line stably expressing a human cytochrome P4501A2 cDNA was developed. Thisrecombinant cell line displayed P4501A2 protein and estradiol2‐hydroxylase activity, neither of which was detectedin the parental cell line. The recombinant cell line was also approximately 1000‐fold more sensitive t o the cyto‐toxicity and mutagenicity of the carcinogenic mycotoxin aflatoxin B1 than was the parent cell line. The increasein mutagenicity was supported by a corresponding increase in the level of aflatoxin B1 binding t o DNA in cellsexpressing P4501A2 relative to control cells.