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Efficient Immortalization by SV40 T DNA of Skin Fibroblasts From Patients With Wilms' Tumor Associated With Chromosome 11p Deletion
Author(s) -
Kano Yoshio,
Little John B.
Publication year - 1989
Publication title -
molecular carcinogenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.254
H-Index - 97
eISSN - 1098-2744
pISSN - 0899-1987
DOI - 10.1002/mc.2940020606
Subject(s) - biology , chromosome , transfection , plasmid , karyotype , microbiology and biotechnology , wilms' tumor , genetics , ploidy , gene , wi 38 , dna , telomere
We report that transfection with a plasmid containing the SV40 early region (T) leads to a very high frequency of immortalization in two different strains of human diploid fibroblasts, each with a partial deletion of the short arm of chromosome 11. Immortalization often occurred without a recognizable “crisis” phase. Preimmortal cells showed a high frequency of spontaneous mutations and chromosomal aberrations. These results suggest that a gene involved in the control of senescence of human cells in vitro may be associated with this chromosome.