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Malignant transformation of human bladder epithelial cells by DNA transfection with the v‐ raf oncogene
Author(s) -
Skouv Jan,
Ottesen Svend,
Mark George,
Autrup Herman
Publication year - 1989
Publication title -
molecular carcinogenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.254
H-Index - 97
eISSN - 1098-2744
pISSN - 0899-1987
DOI - 10.1002/mc.2940020202
Subject(s) - biology , transfection , oncogene , cell culture , microbiology and biotechnology , messenger rna , transformation (genetics) , immortalised cell line , western blot , malignant transformation , northern blot , cancer research , antigen , cell , gene , immunology , genetics , cell cycle
Transfection of the v‐ raf oncogene into immortalized, nontumorigenic human bladder epithelial cells resulted in the isolation of two tumorigenic transformants. Both were identified as human and of the same origin as the parent cell line by human leukocyte antigen typing and Southern blot analysis. Both the primary tumorigenic transfectants and the cell lines established from the induced tumors expressed v‐ raf mRNA and v‐ raf protein. In both tumorigenic transformants the level of c‐ myc mRNA was enhanced compared with that of the parent cell line.