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Matrix metallopeptidase 9 targeted by hsa‐miR‐494 promotes silybin‐inhibited osteosarcoma
Author(s) -
Sun Tianhao,
Cheung Kelvin S. C.,
Liu ZhiLi,
Leung Frankie,
Lu William W.
Publication year - 2018
Publication title -
molecular carcinogenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.254
H-Index - 97
eISSN - 1098-2744
pISSN - 0899-1987
DOI - 10.1002/mc.22753
Subject(s) - biology , viability assay , cancer research , western blot , downregulation and upregulation , cell growth , mtt assay , microbiology and biotechnology , osteosarcoma , matrix metalloproteinase , apoptosis , biochemistry , gene
Osteosarcoma (OS) is the most common malignant tumor that develops in bone. Its mortality is very high. Therefore, study of mechanisms of pathogenesis of the OS is urgently required. Previous studies of microarray showed that the expression levels of matrix metallopeptidase 9 (MMP‐9) altered significantly in OS. In addition, overexpression of MMP‐9 is recognized as an indicator in cancer. However, the exact roles of MMP‐9 in OS are not fully investigated. Thus, we firstly studied the roles of MMP‐9 in OS and revealed that silence of MMP‐9 inhibited OS cell proliferation as determined by MTT assay and colony formation assay. Secondly, we conducted TUNEL assay and confirmed loss of functions of MMP‐9 induced OS cell apoptosis. Next, we used lentivector packaging method to overexpress MMP‐9 and found that overexpression of MMP‐9 promoted OS cell migration. Fourthly, the results of luciferase assay showed that MMP‐9 was targeted by hsa‐miR‐494, which inhibited OS. Fifthly, we revealed that the levels of hsa‐miR‐494 were upregulated by the drug silybin which inhibited OS. Finally, we revealed that silybin inhibited OS cell viability by altering the protein levels of β‐catenin and Runt‐related transcription factor 2 (RUNX2) as determined by western blot and immunocytochemistry (ICC).