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Oroxylin A regulates glucose metabolism in response to hypoxic stress with the involvement of Hypoxia‐inducible factor‐1 in human hepatoma HepG2 cells
Author(s) -
Dai Qinsheng,
Yin Qian,
Wei Libin,
Zhou Yuxin,
Qiao Chen,
Guo Yongjian,
Wang Xiaotang,
Ma Shiping,
Lu Na
Publication year - 2016
Publication title -
molecular carcinogenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.254
H-Index - 97
eISSN - 1098-2744
pISSN - 0899-1987
DOI - 10.1002/mc.22369
Subject(s) - biology , hypoxia (environmental) , microbiology and biotechnology , glycolysis , cancer cell , gene silencing , cell , hypoxia inducible factors , metabolism , pharmacology , biochemistry , cancer , chemistry , gene , oxygen , organic chemistry , genetics
Metabolic alteration in cancer cells is one of the most conspicuous characteristics that distinguish cancer cells from normal cells. In this study, we investigated the influence and signaling ways of oroxylin A affecting cancer cell energy metabolism under hypoxia. The data showed that oroxylin A remarkably reduced the generation of lactate and glucose uptake under hypoxia in HepG2 cells. Moreover, oroxylin A inhibited HIF‐1α expression and its stability. The downstream targets (PDK1, LDHA, and HK II), as well as their mRNA levels were also suppressed by oroxylin A under hypoxia. The silencing or the overexpression of HIF‐1α assays suggested that HIF‐1α is required for metabolic effect of oroxylin A in HepG2 cells during hypoxia. Furthermore, oroxylin A could reduce the expression of complex III in mitochondrial respiratory chain, and then decrease the accumulation of ROS at moderate concentrations (0–50 µM) under hypoxia, which was benefit for its inhibition on glycolytic activity by decreasing ROS‐mediated HIF‐1 expression. Besides, oroxylin A didn't cause the loss of MMP under hypoxia and had no obvious effects on the expression of OXPHOS complexes, suggesting that oroxylin A did not affect mitochondrial mass at the moderate stress of oroxylin A. The suppressive effect of oroxylin A on glycolysis led to a significantly repress of ATP generation, for ATP generation mostly depends on glycolysis in HepG2 cells. This study revealed a new aspect of glucose metabolism regulation of oroxylin A under hypoxia, which may contribute to its new anticancer mechanism. © 2015 Wiley Periodicals, Inc.