Premium
Expression of FOXJ1 in hepatocellular carcinoma: Correlation with patients' prognosis and tumor cell proliferation
Author(s) -
Chen HongWei,
Huang XiaoDong,
Li HongChen,
He Song,
Ni RunZhou,
Chen CuiHua,
Peng Chen,
Wu Gang,
Wang GuiHua,
Wang YingYing,
Zhao YunHong,
Zhang YiXin,
Shen AiGuo,
Wang HuiMin
Publication year - 2013
Publication title -
molecular carcinogenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.254
H-Index - 97
eISSN - 1098-2744
pISSN - 0899-1987
DOI - 10.1002/mc.21904
Subject(s) - biology , cancer research , cell growth , hepatocellular carcinoma , gene knockdown , cell cycle , immunohistochemistry , cell , cell culture , immunology , genetics
FOXJ1 is a member of the forkhead box (FOX) family of transcription factors. Recent studies suggested that FOXJ1 may function as a tumor suppressor gene in breast cancer. To investigate the potential roles of FOXJ1 in hepatocellular carcinoma (HCC), expression of FOXJ1 was first examined in eight paired frozen HCC and adjacent noncancerous liver tissues by Western blot, and we found that FOXJ1 was upregulated in HCC specimens. In addition, immunohistochemistry was performed to confirm our results in 108 HCC samples. Moreover, we also evaluated its relation with clinicopathological variables and the prognostic significance. The data showed that high expression of FOXJ1 was associated with histological grade ( P < 0.001), and FOXJ1 was positively correlated with proliferation marker Ki‐67 ( P < 0.01). Univariate analysis suggested that FOXJ1 expression was associated with poor prognosis ( P < 0.001). Multivariate analysis indicated that tumor grade ( P < 0.0001), metastasis ( P = 0.0451), tumor size ( P = 0.0459), FOXJ1 ( P = 0.0011), and Ki‐67 ( P = 0.0006) were independent prognostic markers for HCC. Furthermore, we noted that there existed the change of the level of FOXJ1 subcellular localization during cell‐cycle transition in HepG2 cells by immunofluorescence and cell fractionation. Besides, we employed FOXJ1 overexpression/knockdown approaches to investigate the effects of FOXJ1 on HCC cell proliferation and cell‐cycle distribution and found that overexpression of FOXJ1 can promote tumor cell proliferation and cell‐cycle transition. Our results suggested that FOXJ1 was overexpressed in HCCs and associated with histological grade and poor prognosis. Overexpression of FOXJ1 was also involved in tumor cell proliferation and cell‐cycle progression in HCC cell lines. © 2012 Wiley Periodicals, Inc.