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Common genetic polymorphisms in pre‐microRNAs and risk of cervical squamous cell carcinoma
Author(s) -
Zhou Bin,
Wang Kana,
Wang Yanyun,
Xi Mingrong,
Zhang Zhu,
Song Yaping,
Zhang Lin
Publication year - 2011
Publication title -
molecular carcinogenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.254
H-Index - 97
eISSN - 1098-2744
pISSN - 0899-1987
DOI - 10.1002/mc.20740
Subject(s) - biology , microrna , single nucleotide polymorphism , allele , genotype , restriction fragment length polymorphism , genetics , gene , pathogenesis , microbiology and biotechnology , cancer research , medicine , oncology , immunology
MicroRNAs (miRNAs) function as gene regulator and they participate in diverse biological pathways. Common single nucleotide polymorphisms (SNPs) in pre‐microRNAs may change their property through altering miRNAs expression and/or maturation. We conducted a pilot study to test whether SNPs in pre‐microRNAs were associated with cervical squamous cell carcinoma (CSCC). Genotypes of three SNPs in pre‐miRNAs ( hsa‐miR‐196a2 rs11614913 C/T, hsa‐miR‐499 rs3746444 A/G, and hsa‐miR‐146a rs2910164 G/C) in 226 CSCC patients and 309 control subjects were determined with the use of PCR‐restriction fragment length polymorphism (RFLP) assay. Significantly increased CSCC risks were found to be associated with G allele of rs3746444 and G allele of rs2910164 ( P = 0.017, OR = 1.454, and P = 0.016, OR = 1.355, respectively). Increased CSCC risks were associated with them in different genetic model ( P = 0.0004, OR = 1.98 for rs3746444 in an overdominant model, and P = 0.024, OR = 2.10 for rs2910164 in a codominant model, respectively). Results of stratified analyses revealed that rs2910164 is associated with tumor differentiation and lymph node status ( P = 0.043, OR = 2.08, and a borderline P = 0.057, OR = 0.41, respectively). No association between rs11614913 and CSCC risk was observed. The present study provides evidence that rs3746444 and rs2910164 are associated with CSCC, indicating that common genetic polymorphisms in pre‐microRNAs contribute to the pathogenesis of CSCC. Mol. Carcinog. © 2011 Wiley‐Liss, Inc.