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Downstream targets of Nm23‐H1: Gene expression profiling of CAL 27 cells using DNA microarray
Author(s) -
Bosnar Maja Herak,
Bago Ružica,
GallTrošelj Koraljka,
Streichert Thomas,
Pavelić Jasminka
Publication year - 2006
Publication title -
molecular carcinogenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.254
H-Index - 97
eISSN - 1098-2744
pISSN - 0899-1987
DOI - 10.1002/mc.20243
Subject(s) - biology , gene expression profiling , gene , downstream (manufacturing) , profiling (computer programming) , microbiology and biotechnology , dna , gene expression , microarray , dna microarray , computational biology , genetics , operations management , computer science , economics , operating system
The human nm23‐H1 was discovered as a tumor metastasis suppressor based on its reduced expression in melanoma cell lines with low versus high metastatic potential. It encodes for one of two subunits of the nucleoside‐diphosphate kinase. Besides its role in the maintenance of the cells NTP pool, nm23 plays a key role in different cellular processes. The role of nm23‐H1 in these processes still has to be elucidated. Our goal was to identify Nm23‐H1 downstream targets by subjecting Nm23‐H1 overexpressing CAL 27 cells oral squamous cell carcinoma (OSSC) to microarray analysis. The genes with changed expression patterns could be clustered into several groups: transforming growth factor β (TGFβ) signaling pathway, cell adhesion, invasion and motility, proteasome machinery, cell‐cycle, epithelial structural and related molecules and others. Based on the expression patterns observed we presume that nm23‐H1 might have a role in OSSCs, which should be confirmed by future experiments. © 2006 Wiley‐Liss, Inc.