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Intestinal microbiota: A potential diet‐responsive prevention target in Apc Min mice
Author(s) -
Mai Volker,
Colbert Lisa H.,
Perkins Susan N.,
Schatzkin Arthur,
Hursting Stephen D.
Publication year - 2007
Publication title -
molecular carcinogenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.254
H-Index - 97
eISSN - 1098-2744
pISSN - 0899-1987
DOI - 10.1002/mc.20233
Subject(s) - lachnospiraceae , biology , gut flora , bacteria , food science , microbiology and biotechnology , firmicutes , biochemistry , genetics , 16s ribosomal rna
We previously reported that two dietary regimens, calorie restriction (CR) and a high olive oil‐containing diet supplemented with a freeze‐dried fruit and vegetable extract (OFV), reduced the development of intestinal adenomas in Apc Min mice by 57% and 33%, respectively, compared to control mice fed a defined diet ad libitum. The OFV diet was designed to have a strong effect on the composition of the intestinal microbiota through its high content of fiber, which represents a major source of fermentable substrate for the gut bacteria. We hypothesized that some of the observed effects of diet on intestinal carcinogenesis might be mediated by diet‐related changes in the bacterial species that thrive in the gut. Therefore, we determined by fluorescent in situ hybridization (FISH) and denaturing gradient gel electrophoresis (DGGE) how the dietary interventions affected the composition of the intestinal microbiota, and we characterized specific microbiota changes that were associated with diet and reduced intestinal carcinogenesis. The OFV diet changed the overall composition of the intestinal microbiota, smaller changes were observed for the CR diet. Furthermore, we detected a 16S rDNA fragment associated with mice that did not develop polyps. Sequence analysis suggested that hitherto unidentified bacteria belonging to the family Lachnospiraceae (order Clostridiales ) were its source. Thus, these bacteria may be an indicator of intestinal conditions associated with reduced intestinal carcinogenesis in Apc Min mice. © 2006 Wiley‐Liss, Inc.