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Mitogen‐regulated protein/proliferin mRNA induction following single applications of tumor promoters to murine skin
Author(s) -
Parfett Craig L.J.
Publication year - 2005
Publication title -
molecular carcinogenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.254
H-Index - 97
eISSN - 1098-2744
pISSN - 0899-1987
DOI - 10.1002/mc.20110
Subject(s) - microbiology and biotechnology , biology , messenger rna , gene expression , northern blot , ornithine decarboxylase , promoter , gene , rna , biochemistry , enzyme
Mitogen‐regulated protein/proliferin ( mrp/plf ) gene family transcripts rise in abundance as a response to diverse chemical and physical agents that promote morphological transformation in the murine C3H/10T1/2 cultured cell model of multi‐step carcinogenesis. To determine if proliferin genes respond to tumor promoters in vivo, RNA was extracted from the whole skin of SENCAR mice after single applications of 2 or 20 μg 12‐ O ‐tetradecanoylphorbol‐13‐acetate (TPA); 3.2 or 32 nmole), 20 or 40 mg benzoyl peroxide (BPO; 83, 165 μmole), or acetone vehicle alone (2.72 mmole). RNA samples were prepared from treated skin areas, 2–48 h after painting. Mrp/plf ‐mRNA was not detected in Northern blot hybridizations, but large increases in mRNAs for ornithine decarboxylase gene and mRNA ( odc ), v‐jun oncogene‐related transcription factor gene and mRNA ( junB ), egr1 (early growth response protein gene and mRNA) were measured relative to beta 2 microglobulin gene and mRNA ( b2m ) mRNA in response to TPA. BPO induced small relative changes in these mRNAs. Reverse transcriptase (RT)‐polymerase chain reactions (PCR) detected fully‐processed MRP/plf ‐mRNA 16–48 h after TPA treatments in five of six animals, and in three of six BPO‐treated animals. The MRP/plf ‐mRNA species expressed in the skin were predominantly plf1 and mrp3 as determined by gene‐specific restriction enzyme sites within the RT‐PCR products. Expression was either undetectable or found at low levels in acetone‐painted controls and was not detected during the anagen phase of the normal hair growth cycle in unpainted animals. These results demonstrate that mrp/plf ‐mRNA is differentially expressed in murine skin in response to mechanistically distinct tumor promoters and has potential utility as a short‐term biomarker for tumor promoting effects in chemical carcinogenesis. © 2005 Government of Canada.