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Plumbagin induces reactive oxygen species, which mediate apoptosis in human cervical cancer cells
Author(s) -
Srinivas Priya,
Gopinath Gokul,
Banerji Asoke,
Dinakar Abhilash,
Srinivas Gopal
Publication year - 2004
Publication title -
molecular carcinogenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.254
H-Index - 97
eISSN - 1098-2744
pISSN - 0899-1987
DOI - 10.1002/mc.20031
Subject(s) - plumbagin , apoptosis , biology , cytochrome c , caspase , reactive oxygen species , programmed cell death , dna fragmentation , microbiology and biotechnology , caspase 9 , cancer cell , ampacity , caspase 3 , biochemistry , cancer , genetics , geometry , mathematics , conductor
There is an emerging evidence that plumbagin (5‐hydroxy‐2‐methyl‐1, 4‐naphthoquinone) may have potential as a chemotherapeutic agent. However, the growth inhibitory mechanisms of plumbagin have remained unexplored. The aim of the study was to determine whether plumbagin‐induced cell death in human cervical cancer cell line, ME‐180, exhibited biochemical characteristics of apoptosis and to check whether N ‐acetyl‐ l ‐cysteine (NAC), which is a free radical scavenger, can reverse the cytotoxic effects of plumbagin. It can be concluded from the results that plumbagin inhibits the growth of ME‐180 cells in a concentration and time‐dependent manner. The cytotoxic effect of plumbagin induced cell death is through the generation of reactive oxygen species (ROS) and subsequent induction of apoptosis as demonstrated by the present data. Treatment of cells with plumbagin caused loss of mitochondrial membrane potential (ΔΨ m ), and morphological changes characteristic of apoptosis, such as the translocation of phosphatidyl serine, nuclear condensation, and DNA fragmentation. Moreover, plumbagin‐induced apoptosis involved release of mitochondrial cytochrome c and apoptosis inducing factor (AIF), thus activation of caspase‐dependent and ‐independent pathways, as shown by the plumbagin‐mediated activation of caspase‐3 and ‐9. Our results also show that pretreatment of ME‐180 cells with NAC blocks plumbagin‐induced loss of ΔΨ m and subsequent release of cytochrome c, AIF, and caspase‐9 and ‐3 activation, thus inhibiting the apoptotic ability of plumbagin. © 2004 Wiley‐Liss, Inc.