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A comprehensive analysis of loss of heterozygosity caused by hemizygous deletions in renal cell carcinoma using a subtraction library
Author(s) -
Hatano Naoya,
Nishikawa Naoko S.,
McElgunn Cathal,
Sarkar Shubhashish,
Ozawa Kazuo,
Shibanaka Yasuhiko,
Nakajima Motowo,
Gohiji Kazuo,
Kiyama Ryoiti
Publication year - 2001
Publication title -
molecular carcinogenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.254
H-Index - 97
eISSN - 1098-2744
pISSN - 0899-1987
DOI - 10.1002/mc.1051
Subject(s) - loss of heterozygosity , biology , genetics , renal cell carcinoma , microsatellite , chromosome , microbiology and biotechnology , gene , allele , pathology , medicine
Several new loci were identified by a comprehensive analysis of loss of heterozygosity (LOH) using a subtraction library between matched normal and renal cell carcinoma (RCC) tissues. A total of 187 clones from the library, with a complexity of 1×10 4 , were mapped, and 44 clusters of the mapped loci were subjected to LOH analysis using microsatellite markers. A total of 27 loci, which exhibited frequencies of LOH of at least 10% among 44 tumors, mostly clear‐cell RCC, included several loci that were reported previously, such as, the von Hippel‐Lindau gene, adenomatous polyposis coli, and interferon regulatory factor‐1, as well as new loci, at 5q32‐q34, 6q21‐q22, 8p12, and others. These loci exhibited LOH among 11.8–93.8% of tumors, and most, if not all, were derived from the sites of hemizyous deletions. The minimum regions of LOH of chromosomes 5, 6, and 8 were 9.0, 10.3, and 0.775 Mb, respectively. The average distance between the cloned fragments on the chromosomes was 2.2 Mb in 187 clones, indicating that the minimum LOH size expected from this subtraction analysis was roughly 50 kb. Therefore, the strategy described here provides comprehensive analysis of LOH sites, which were mostly caused by hemizygous deletions. © 2001 Wiley‐Liss, Inc.