Premium
Proteomic analysis of human prostate cancer †
Author(s) -
Ahram Mamoun,
Best Carolyn J. M.,
Flaig Michael J.,
Gillespie John W.,
Leiva Isabel M.,
Chuaqui Rodrigo F.,
Zhou Ge,
Shu Hungjun,
Duray Paul H.,
Linehan W. Marston,
Raffeld Mark,
Ornstein David K.,
Zhao Yingming,
Petricoin Emanuel F.,
EmmertBuck Michael R.
Publication year - 2002
Publication title -
molecular carcinogenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.254
H-Index - 97
eISSN - 1098-2744
pISSN - 0899-1987
DOI - 10.1002/mc.10019
Subject(s) - biology , prostate cancer , carcinogenesis , laser capture microdissection , proteomics , proteome , microdissection , prostate , gene expression profiling , computational biology , microarray analysis techniques , microarray , cancer research , cancer , gene , microbiology and biotechnology , bioinformatics , gene expression , genetics
Proteomics is a promising approach in the identification of proteins and biochemical pathways involved in tumorigenesis. In an effort to discover such proteins and pathways that are deregulated in prostate tumorigenesis, cellular proteomes of matched normal prostate epithelial cells and high‐grade prostate cancer cells were analyzed by tissue microdissection, two‐dimensional electrophoresis, and mass spectrometry. Forty protein alterations were detected in the tumors; however, the majority of these changes were not shared among the 12 neoplasms. In contrast, parallel cDNA microarray analysis identified a number of common gene expression changes. The marked heterogeneity of the observed protein alterations may have significance with regard to tumor biology and research strategies for molecular profiling analyses of human prostate cancer. Published 2002 Wiley‐Liss, Inc.