Open AccessCulture of attached and suspended Rhodopseudomonas faecalis in the presence of decomposing fish feed
Author(s) -
Wang Xiaodong,
Liu Xingguo,
Lu Shimin,
Liu Chong,
Gu Zhaojun,
Zeng Xianlei,
Ni Qi
Publication year - 2019
Publication title -
microbiologyopen
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.881
H-Index - 36
ISSN - 2045-8827
DOI - 10.1002/mbo3.924
Subject(s) - rhodopseudomonas palustris , tap water , rhodopseudomonas , food science , biomass (ecology) , chemistry , phosphorus , bacteria , biology , botany , environmental engineering , ecology , photosynthesis , environmental science , genetics , organic chemistry
Abstract An approach to culturing attached and suspended forms of Rhodopseudomonas faecalis by using compound fish feed with tap water in transparent containers is reported in this study. The ratio of fish feed to tap water was 14.3–50.8 g/L, and no other inoculum or substances were added during the culture process. When the ratio of fish feed to tap water was 14.3 g/L, the highest total nitrogen, total phosphorus, and total dissolved carbon content recorded in the water in the containers were approximately 730 mg/L, 356 mg/L, and 1,620 mg/L, respectively, during the process of feed decay. Comamonas, Rhodopseudomonas, and Clostridium successively dominated during the culture process. Rhodopseudomonas was the most common dominant genus in both the attached and suspended forms when the water was dark red, and the relative operational taxonomic unit abundance reached 80‒89% and 24.8%, respectively. The dominant species was R. faecalis . The maximum thickness of attached bacteria and the biomass of attached Rhodopseudomonas reached up to 0.56 mm and 7.5 mg/cm 2 , respectively. This study provides a method for the mass culture of Rhodopseudomonas by using the fermentation of aquatic compound fish feed.