Open AccessCyclohexane, naphthalene, and diesel fuel increase oxidative stress, CYP153 , sodA, and recA gene expression in Rhodococcus erythropolis
Author(s) -
Sazykin Ivan,
Makarenko Maksim,
Khmelevtsova Ludmila,
Seliverstova Ekaterina,
Rakin Alexander,
Sazykina Marina
Publication year - 2019
Publication title -
microbiologyopen
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.881
H-Index - 36
ISSN - 2045-8827
DOI - 10.1002/mbo3.855
Subject(s) - superoxide dismutase , superoxide , chemistry , oxidative stress , hydrogen peroxide , biochemistry , reactive oxygen species , hydrocarbon , naphthalene , gene expression , photochemistry , gene , enzyme , organic chemistry
In this study, we compared the expression of CYP153 , sodA , sodC, and recA genes and ROS generation in hydrocarbon‐degrading Rhodococcus erythropolis in the presence of cyclohexane, naphthalene, and diesel fuel. The expression of cytochrome P450, sodA (encoding Fe/Mn superoxide dismutase), recA, and superoxide anion radical generation rate increased after the addition of all studied hydrocarbons. The peak of CYP153 , sodA, and recA gene expression was registered in the presence of naphthalene. The same substrate upregulated the Cu/Zn superoxide dismutase gene, sodC . Cyclohexane generated the highest level of superoxide anion radical production. Hydrogen peroxide accumulated in the medium enriched with diesel fuel. Taken together, hydrocarbon biotransformation leads to oxidative stress and upregulation of antioxidant enzymes and CYP153 genes, and increases DNA reparation levels in R. erythropolis cells.