Open AccessA survey of antimicrobial resistance in Enterobacteriaceae isolated from the Chesapeake Bay and adjacent upper tributaries
Author(s) -
Riedel Stefan,
Boire Nicholas,
Carson Kathryn A.,
Vadlamudi Aravinda,
Khuvis Joshua,
Vadlamudi Vivek,
Atukorale Vajini,
Riedel Victoria A. A.,
Parrish Nicole M.
Publication year - 2019
Publication title -
microbiologyopen
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.881
H-Index - 36
ISSN - 2045-8827
DOI - 10.1002/mbo3.839
Subject(s) - serratia marcescens , enterobacter aerogenes , microbiology and biotechnology , antibiotic resistance , enterobacter cloacae , biology , antimicrobial , klebsiella pneumoniae , enterobacter , serratia , acinetobacter , enterobacteriaceae , tetracycline , escherichia coli , bacteria , antibiotics , pseudomonas , biochemistry , genetics , gene
In recent years, the rise in antimicrobial resistance (AR) in the healthcare setting as well as the environment has been recognized as a growing public health problem. The Chesapeake Bay (CB) and its upper tributaries (UT) is a large and biologically diverse estuary. This pilot study evaluated the presence of AR of gram‐negative bacteria isolated from water samples collected at various sites of the Chesapeake Bay. Bacterial organisms were identified and antimicrobial susceptibility testing was performed by phenotypic and genotypic methods. Ninety‐two distinctly different gram‐negative bacteria were identified; Klebsiella pneumoniae , Enterobacter cloacae , Enterobacter aerogenes , Serratia marcescens , and Escherichia coli were most often isolated. Serratia marcescens was more frequently isolated in samples from the UT compared to the CB. Antimicrobial resistance was more frequently detected in organisms from the CB by phenotypic and genotypic methods. Antimicrobial resistance to ampicillin, imipenem, tetracycline, and chloramphenicol were the most frequently observed resistance patterns. ACT‐1, CMY, and SHV genes were the most frequently detected resistance genes, with predominance in organism isolated from the CB. The results from this study emphasize the importance for further developing comprehensive surveillance programs of AR in bacterial isolates in the various environments, such as recreational and other water systems.