Open AccessMagnetic immobilization of a quorum sensing signal hydrolase, AiiA
Author(s) -
Wang Lin,
Xu Haixing,
Liu Zewen,
Sun Taolei,
Yuan Chengqing,
Yang Ying,
Guo Junhui,
Xie Hao
Publication year - 2019
Publication title -
microbiologyopen
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.881
H-Index - 36
ISSN - 2045-8827
DOI - 10.1002/mbo3.797
Subject(s) - quorum sensing , biofilm , quorum quenching , chemistry , hydrolase , escherichia coli , microbiology and biotechnology , signal (programming language) , enzyme , antimicrobial , biophysics , biochemistry , bacteria , biology , computer science , genetics , gene , programming language
Magnetic immobilization of quorum sensing (QS) signal hydrolases provides a convenient solution for quenching QS process that is essential for bacterial biofilm formation and antimicrobial resistance. In the present study, a QS signal hydrolase, AiiA, was fused with a magnetic protein, MagR, and expressed in Escherichia coli . Magnetic immobilization of AiiA was achieved on Fe 3 O 4 ‐SiO 2 iron beads and was confirmed via SDS‐PAGE, zeta potential measurement, FTIR spectrometry, and SEM analysis. The magnetic immobilized AiiA exhibited activity in degrading the quorum sensing signal, C6‐HSL. This study opens a new avenue to actively immobilize enzymes via magnetic interaction and quench quorum sensing.