Open AccessAquella oligotrophica gen. nov. sp. nov.: A new member of the family Neisseriaceae isolated from laboratory tap water
Author(s) -
Chan KokGan,
SeeToo WahSeng,
Chua KahOoi,
Peix Álvaro,
Goh Kian Mau,
Hong KarWai,
Yin WaiFong,
Lee LiSin
Publication year - 2019
Publication title -
microbiologyopen
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.881
H-Index - 36
ISSN - 2045-8827
DOI - 10.1002/mbo3.793
Subject(s) - biology
A bacterial strain designated as P08 T was isolated from laboratory tap water during a water quality assessment in University of Malaya, Malaysia. The strain was a Gram‐negative, rod‐shaped, nonmotile, and aerobic bacterium. Complete genome of P08 T comprised of a 2,820,660 bp chromosome with a G + C content of 36.43%. Both 16S rRNA phylogeny and phylogenetic tree inferred from the core gene matrix demonstrated that P08 T formed a hitherto unknown subline within the family Neisseriaceae . Ortho average nucleotide identity (OrthoANI) values and the percentage of conserved proteins (POCP) calculated from complete genome sequence indicated low relatedness between P08 T and its phylogenetic neighbors. Respiratory quinone analysis revealed Q‐8 as the only detectable quinone. The predominant cellular fatty acids were identified as C 14:0 , iso‐C 15:0 , and summed feature 3 (C 16:1 ω 7c/C 16:1 ω 6c). The polar lipids consisted of uncharacterized aminolipid, phosphatidylglycerol, and phosphatidylethanolamine. All aspects of phenotypic and phylogenetic data suggested that strain P08 T represents a novel genus within family Neisseriaceae , for which the name Aquella gen. nov. is proposed. The type species of the genus is Aquella oligotrophica sp. nov., and the type strain is P08 T (=LMG 29629 T =DSM 100970 T ).