Open AccessAncrocorticia populi gen. nov., sp. nov, isolated from the symptomatic bark of Populus × euramericana canker
Author(s) -
Xu Guantang,
Xue Han,
Piao Chungen,
Guo Minwei,
Li Yong
Publication year - 2019
Publication title -
microbiologyopen
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.881
H-Index - 36
ISSN - 2045-8827
DOI - 10.1002/mbo3.792
Subject(s) - bark (sound) , traditional medicine , botany , biology , geography , forestry , medicine
A Gram‐staining positive facultative anaerobic, non‐motile strain, sk1b4 T , was isolated from canker of symptomatic bark tissue of a Populus × euramericana . 16S rRNA gene sequence analyses showed that strain sk1b4 T shared the highest similarity with Arcanobacterium phocisimile (94.1%). Within the phylogenetic tree, the novel isolate formed a distinct branch from Actinobaculum , Arcanobacterium , and Trueperella . The percentage of conserved proteins calculated from genomic sequence indicated a low level of relatedness between the novel strain and its phylogenetic neighbors. Growth of the novel strain occurred at temperatures between 10 and 41°C, and within a pH range of 6.0–9.0; optimal growth occurred at 30°C and at pH 6.0–9.0. Growth also occurred within a NaCl concentration of 1%–5% (w/v). The major fatty acids of the strain were C 14:0 , C 16:0 , and C 18:1 ω 9 c , and major polar lipids were glycolipid, phosphatidylinositol mannoside, phospholipid, diphosphatidylglycerol, and phosphatidylglycerol. Respiratory quinone was absent. On the basis of phenotypic and genotypic characteristics, we propose that the novel isolate should be classified as a novel species in a new genus: Ancrocorticia populi gen. nov., sp. nov. The type strain is sk1b4 T (=CFCC 14564 T = KCTC 39919 T ).