Open AccessGenome sequence of Methylocystis hirsuta CSC1, a polyhydroxyalkanoate producing methanotroph
Author(s) -
Bordel Sergio,
Rodríguez Elisa,
Muñoz Raúl
Publication year - 2019
Publication title -
microbiologyopen
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.881
H-Index - 36
ISSN - 2045-8827
DOI - 10.1002/mbo3.771
Subject(s) - polyhydroxyalkanoates , methanotroph , contig , whole genome sequencing , biology , genome , plasmid , operon , genetics , gene , bacteria , biochemistry , anaerobic oxidation of methane , mutant , catalysis
Abstract Polyhydroxyalkanoates (PHAs) are biodegradable plastics that can be produced by some methanotrophic organisms such as those of the genus Methylocystis . This allows the conversion of a detrimental greenhouse gas into an environmentally friendly high added‐value bioproduct. This study presents the genome sequence of Methylocystis hirsuta CSC1 (a high yield PHB producer). The genome comprises 4,213,043 bp in 4 contigs, with the largest contig being 3,776,027 bp long. Two of the other contigs are likely to correspond to large size plasmids. A total of 4,664 coding sequences were annotated, revealing a PHA production cluster, two distinct particulate methane monooxygenases with active catalytic sites, as well as a nitrogen fixation operon and a partial denitrification pathway.